Effect of sensory neuropeptide substance P on the differentiation of cultured epidermal stem cells in vitro / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 295-298, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-303727
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of sensory neuropeptide substance P (SP) on the differentiation of cultured epidermal stem cells (ESC) in vitro,with in vitro cultured ESC as the platform.</p><p><b>METHODS</b>ESC from newborn Wistar rats were isolated, purified by repeated passages in culture. SP was added for stimulation when ESC clone grew. Immunohistochemistry staining with K14 antibody, and flow cytometry (FCM) was performed at 0, 24th, 48th, 72nd, 96th, 144th, 192nd, 240th, 288th, 336th, 384th, 432nd post differentiation hours (PDH) to identify the cell groups and to detect if there were transient amplifying cells (TAC) among the cells.</p><p><b>RESULTS</b>ESC in culture formed large colonies after SP treatment with positive staining for K14, indicating that they were TACs. The results of FCM indicated that when ESC were stimulated by SP, TAC colony formation occurred and the cell number increased in a constant speed.</p><p><b>CONCLUSION</b>ESC could differentiate into TAC by neuropeptide SP induction, and the number of ESC kept on a certain level during the process.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Sensory Receptor Cells
/
Stem Cells
/
Substance P
/
Cell Differentiation
/
Cells, Cultured
/
Chemistry
/
Rats, Wistar
/
Cell Biology
/
Endothelial Cells
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2004
Type:
Article
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