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Expression pattern of Mycobacterium tuberculosis Ag85B and its value in pathological diagnosis / 中华病理学杂志
Chinese Journal of Pathology ; (12): 600-603, 2014.
Article in Chinese | WPRIM | ID: wpr-304441
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of Mycobacterium tuberculosis secreted protein Ag85B in paraffin-embedded tissues by immunohistochemistry (IHC), and to evaluate its application in the pathological diagnosis of tuberculosis.</p><p><b>METHODS</b>One hundred and five tuberculosis specimens (54 pulmonary tuberculosis, 51 lymph nodal tuberculosis) and 51 specimens of other diseases (8 lung cancer, 10 pulmonary abscess, 10 bronchiectasis, 7 lymphoma, 5 necrotizing lymphadenitis, 4 reactive hyperplasia lymphoid, and 7 sarcoidosis) were collected from January 2012 to July 2013 from Beijing Chest Hospital, Capital Medical University. One-step IHC was performed on paraffin-embedded tissues using antibody directed against Ag85B.</p><p><b>RESULTS</b>IHC and Ziehl-Neelsen (ZN) acid-fast staining showed that distribution and intensity of Ag85B expression were concordant with the distribution and number of acid-fast bacilli. IHC showed significantly higher sensitivity than ZN staining (50.5%, 53/105 vs. 31.4%, 33/105; χ² = 7.877, P = 0.005). The combined sensitivity of IHC and ZN staining was 59.0%. Moreover, oil immersion was not necessary for IHC, allowing more rapid diagnosis.</p><p><b>CONCLUSION</b>IHC detection of Ag85B is a simple method with higher sensitivity than ZN staining, and demonstrated good value in the pathological diagnosis of tuberculosis.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Sarcoidosis / Staining and Labeling / Tuberculosis, Lymph Node / Tuberculosis, Pulmonary / Bronchiectasis / Acyltransferases / Immunohistochemistry / Biomarkers / Diagnosis / Allergy and Immunology Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Pathology Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Sarcoidosis / Staining and Labeling / Tuberculosis, Lymph Node / Tuberculosis, Pulmonary / Bronchiectasis / Acyltransferases / Immunohistochemistry / Biomarkers / Diagnosis / Allergy and Immunology Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Pathology Year: 2014 Type: Article