Chromosomal translocation involving USP6 gene in nodular fasciitis / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 533-536, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-304458
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the frequency of USP6 gene rearrangement in nodular fasciitis (NF) and to evaluate its clinical application.</p><p><b>METHODS</b>Twenty nine cases of previously diagnosed NF were screened for the presence of the USP6 gene rearrangement by interphase fluorescence-in-situ hybridization (FISH) on formalin-fixed paraffin-embedded tissue. Fifteen of these cases, which had available tissue, were also analysed for MYH9-USP6 fusion transcripts by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Twenty four of the 29 cases (83%) were positive for the USP6 gene rearrangement by interphase FISH. The 15 cases with RT-PCR showed the following results:
11 positive, one deletion and three negative for USP6 gene rearrangement. Of these 15 cases, eight (8/15) showed MYH9-USP6 fusion transcript by RT-PCR. Of these eight cases, seven were positive for USP6 gene rearrangement and one showed USP6 deletion by FISH.</p><p><b>CONCLUSIONS</b>USP6 gene rearrangement is a recurrent genetic event in NF. It is a valuable ancillary tool for the pathological diagnosis of these lesions.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Translocation, Genetic
/
Gene Rearrangement
/
Proto-Oncogene Proteins
/
In Situ Hybridization, Fluorescence
/
Reverse Transcriptase Polymerase Chain Reaction
/
Ubiquitin Thiolesterase
/
Fasciitis
/
Genetics
/
Interphase
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2014
Type:
Article
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