Effects of dilution rate and carbon-to-nitrogen ratio on lipid accumulation by Rhodosporidium toruloides under chemostat conditions / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 56-64, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-304513
ABSTRACT
The objective of this work is to investigate how dilution rate and carbon-to-nitrogen (C/N) ratio affects lipid accumulation by Rhodosporidium toruloides AS 2.138 9 in continuous culture. Under steady-state conditions, the increase in dilution rate led to the decrease in lipid content and lipid yield. The highest lipid yield and lipid content at D = 0.02 h(-1) were 0.18 g lipid/g sugar and 57.1%, respectively, while the highest lipid productivity and biomass productivity were obtained at D = 0.14 h(-1). The increase in C/N ratio led to the increase in lipid content. The highest lipid content of 38% was obtained at C/N = 237. The highest lipid yield of 0.12 g lipid/g sugar was obtained at C/N = 92. However, the highest lipid productivity of 0.12 g/(L x h) was obtained at C/N = 32. No significant changes were observed in terms of fatty acid composition of the lipid produced under different C/N ratios, and these three fatty acids, palmitic acid, stearic acid and oleic acid, took over 85% in all samples.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Basidiomycota
/
Carbon
/
Palmitic Acid
/
Oleic Acid
/
Culture Media
/
Fatty Acids
/
Batch Cell Culture Techniques
/
Glucose
/
Lipids
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS