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Strategy for soluble expression of phage-displayed scFv antibody specific for zebrafish vitellogenin / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1637-1644, 2011.
Article in Chinese | WPRIM | ID: wpr-304536
ABSTRACT
We developed a new method for soluble expression of phage-displayed scFv antibody specific for zebrafish vitellogenin. The scFv antibody F5 could bind zebrafish vitellogenin specifically in phage-displayed form but not soluble form. The gene of scFv antibody F5 was cloned into vector pET 32a and transferred into Escherichia coli ori DE3. With inducible expression, soluble scFv antibody 32a-F5 was obtained successfully and could also specifically bind to zebrafish vitellogenin. The insoluble expression of phage-displayed scFv antibody was a common problem in the practical use of phage display. This study offered a feasible way to express soluble scFv antibodies with biological activity.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Solubility / Zebrafish / Recombinant Proteins / Molecular Sequence Data / Base Sequence / Vitellogenins / Amino Acid Sequence / Allergy and Immunology / Escherichia coli / Single-Chain Antibodies Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Solubility / Zebrafish / Recombinant Proteins / Molecular Sequence Data / Base Sequence / Vitellogenins / Amino Acid Sequence / Allergy and Immunology / Escherichia coli / Single-Chain Antibodies Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2011 Type: Article