Progress in improvement of lentiviral vectors' transcriptional read-through / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1541-1548, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-304547
ABSTRACT
Four out of 10 patients of X-linked severe combined immunodeficiency (X-SCID) were finally developed leukemia after receiving the treatment of gene therapy delivered by gamma-retroviral vectors. This is due to the vector integrated to the proximity of lmo2 etc proto-oncogene promoters, leading to the activation of onco-gene expression, which raises the concern of the bio-safety of gene therapy vectors. Lentiviral vectors, especially self-inactivating lentiviral vectors, are considered to be much safer than gamma-retroviral vectors. However self-inactivating lentiviral vectors also have encountered with some unsafe factors and one of them is the problem of transcriptional "read-through" . During the past years, achievements have been made to reduce lentiviral vector transcriptional read-through, which are reviewed herein.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription, Genetic
/
Genetic Therapy
/
Proto-Oncogene Proteins
/
Promoter Regions, Genetic
/
Virus Integration
/
Lentivirus
/
Virus Inactivation
/
Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article
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