Establishment of RAW264.7 cell line stably expressing Mycobacterium tuberculosis protein ESAT-6 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1390-1396, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-304564
ABSTRACT
For studying the effects of Mycobacterium tuberculosis secretory protein ESAT-6 on the related functions of macrophages, RAW264.7 cells were transfected with pEGFP-C1-ESAT-6 and pEGFP-C1 by liposome respectively. After screening with a high level of G418, the macrophage cell lines that stably expressed EGFP-ESAT-6 fusion protein or EGFP were established. The gene and protein expression levels were further analyzed by RT-PCR, fluorescence microscopy and Western blotting. The results indicated that the EGFP-ESAT6 fusion gene was integrated into the chromosome and the protein could be stably expressed in the selected macrophage cell line. These results gave us a tool for the future study in the mechanisms of ESAT-6 protein in modulating the macrophage cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacterial Proteins
/
Recombinant Fusion Proteins
/
Transfection
/
Cell Line
/
Cell Biology
/
Green Fluorescent Proteins
/
Genetics
/
Macrophages
/
Metabolism
/
Antigens, Bacterial
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article
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