Screening and identification of marine fungi against bacterial quorum sensing / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1337-1346, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-304570
ABSTRACT
The discovery of quorum sensing (QS) system and its critical role in bacterial virulence have revealed a new way to attack pathogenic bacterium. The pathogenecity of QS deletion mutants decreases significantly. Targeting bacterial QS system is a promising therapeutic approach to control infections and anti-microbial resistance. To obtain natural QS inhibitors from marine organisms, marine fungi (69 strains) were isolated from marine mollusca, and their extracts were screened using improved QSIS2 (Quorum Sensing Inhibitor Selector 2) assay and Chromobacterium violaceum CV026. To improve the efficiency of QSIS2 screening, 2,3,5-triphenyltetrazolium chloride (TTC) staining method was used. Extract from strain QY013 was found to have QS inhibitory activity. Further experiment indicated that pyocyanin in Pseudomonas aeruginosa PAOI and violacein in C. violaceum CV026 were reduced by QY013 extract, without affecting bacterial growth. Morphological and 18S rDNA sequence analysis revealed that strain QY013 was most closely related to Penicillium species. The above results suggest that active constituents from QY013 may be used as novel antimicrobial agents against bacterial infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penicillium
/
Pharmacology
/
Physiology
/
Pseudomonas aeruginosa
/
Virulence
/
Bacterial Physiological Phenomena
/
Quorum Sensing
/
Fungi
/
Marine Biology
/
Metabolism
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article
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