Relationship between glucocorticoid receptors and glucocorticoid resistance in children with idiopathic thrombocytopenia purpura / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 714-716, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-304606
ABSTRACT
<p><b>OBJECTIVE</b>To identify the relationship between the expression of alpha and beta-isoforms of glucocorticoid receptors (GR) in peripheral blood mononuclear cells (PBMC) and glucocorticoid resistance in children with idiopathic thrombocytopenia purpura (ITP).</p><p><b>METHODS</b>Real-time PCR was used to detect the expression of GR alpha and GR beta mRNA in PBMC from 30 children with ITP (glucocorticoid-sensitive, n=18; glucocorticoid-resistant, n=12) and 10 healthy children (control group). Enzyme immunoassay was used to measure plasma levels of total glucocorticoids.</p><p><b>RESULTS</b>There were no significant differences in PBMC GR alpha mRNA levels among the glucocorticoid sensitive, the glucocorticoid-resistant and the control groups. Compared with the glucocorticoid-sensitive and the control groups, the expression of GR beta mRNA in the glucocorticoid-resistant group was significantly up-regulated (p<0.01). Plasma total glucocorticoids level in the glucocorticoid-resistant group was found to be much higher than that in the glucocorticoid-sensitive and the control groups (p<0.01).</p><p><b>CONCLUSIONS</b>The up-regulated expression of GR beta mRNA may associated with glucocorticoid resistance in children with ITP.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Blood
/
Drug Resistance
/
RNA, Messenger
/
Receptors, Glucocorticoid
/
Purpura, Thrombocytopenic, Idiopathic
/
Drug Therapy
/
Genetics
/
Glucocorticoids
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2009
Type:
Article
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