Your browser doesn't support javascript.
loading
Effects of single heat stress treatment on spermatogenic cells in mice / 中华男科学杂志
National Journal of Andrology ; (12): 6-11, 2016.
Article in Chinese | WPRIM | ID: wpr-304759
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of single heat stress treatment on spermatogenic cells in mice.</p><p><b>METHODS</b>We randomly divided 36 C57 male mice into a control and a heat stress treatment group and submerged the lower part of the torso in water at 25 °C and 43 °C, respectively, both for 15 minutes. At 1, 7, and 14 days after treatment, we obtained the testicular organ indexes, observed the changes in testicular morphology by HE staining, and determined the location and expression levels of the promyelocytic leukemia zinc finger (PLZF) and synaptonemal comlex protein-3 (SCP-3) in the testis tissue by immunohistochemistry and Western blot.</p><p><b>RESULTS</b>The testicular organ index was significantly lower in the heat stress treatment than in the control group (P < 0.05). Compared with the controls, the heat shock-treated mice showed loosely arranged spermatogenic cells scattered in the seminiferous tubules at 1 day after heat stress treatment, atrophied, loosely arranged and obviously reduced number of spermatogenic cells at 7 days, and relatively closely arranged seminiferous tubules and increased number and layers of spermatogenic cells at 14 days. The number of SCP-3 labelled spermatocytes obviously decreased in the heat stress-treated animals at 1 and 7 days and began to increase at 14 days. The PLZF protein expression was significantly reduced in the heat stress treatment group at 1 day as compared with that in the control (0.19 ± 0.12 vs 0.64 ± 0.03, P < 0.01), but elevated to 0.77 ± 0.02 at 7 and 14 days, even remarkably higher than in the control animals (P < 0.01).</p><p><b>CONCLUSION</b>Heat stress treatment can induce short-term dyszoospermia in mice, which can be recovered with the prolonged time after treatment.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Seminiferous Tubules / Spermatocytes / Testis / Transcription Factors / Immunohistochemistry / Nuclear Proteins / Blotting, Western / Cell Biology / Tumor Suppressor Proteins Limits: Animals Language: Chinese Journal: National Journal of Andrology Year: 2016 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Seminiferous Tubules / Spermatocytes / Testis / Transcription Factors / Immunohistochemistry / Nuclear Proteins / Blotting, Western / Cell Biology / Tumor Suppressor Proteins Limits: Animals Language: Chinese Journal: National Journal of Andrology Year: 2016 Type: Article