Successful pregnancy following laser-assisted selection of viable but immotile spermatozoa for intracytoplasmic sperm injection: A report of 2 cases / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the feasibility and clinical application value of selecting viable spermatozoa by noncontact diode laser.</p><p><b>METHODS</b>We obtained immotile spermatozoa from 2 infertile men with obstructive azoospermia or severe asthenospermia and selected viable spermatozoa using a single laser shot at the sperm tail. Those that responded to the laser shot by a curling reaction of the tail were regarded as presumably viable and used for intracytoplasmic sperm injection (ICSI).</p><p><b>RESULTS</b>The mean fertilization rate was 88.89% after ICSI with the laser-selected viable spermatozoa. Both of the embryo transfers resulted in a single pregnancy.</p><p><b>CONCLUSION</b>Noncontact diode laser is a useful alternative for the assessment of sperm viability, which may help to achieve successful pregnancy.</p>