Inhibitory effect of Genipin on uncoupling protein-2 and energy metabolism of androgen-independent prostate cancer cells / 中华男科学杂志
National Journal of Andrology
;
(12): 973-976, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-304788
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether the inhibitory effect of Genipin on uncoupling protein-2 (UCP-2) in mitochondria is involved in energy metabolism of androgen-independent PC3 prostate cancer cells.</p><p><b>METHODS</b>PC3 prostate cancer cells were cultured and treated with Genipin at the concentrations of 40, 80, and 160 μmol/L for 48 hours. Then the proliferation of the cells was detected by MTT assay, the expression of UCP-2 mRNA determined by RT-PCR, and the content of intracellular pyruvic acid (PA) and the activity of succinate dehydrogenase (SDH) in the mitochondria measured by visible spectrophotometry.</p><p><b>RESULTS</b>With the increased concentration of Genipin, the proliferative activity of the PC-3 cells, the expression level of UCP-2 mRNA, the content of intracellular PA and the activity of SDH in the cells were all decreased, namely, with the enhanced inhibitory effect of Genipin on UCP-2, a trend of reduction was observed in the proliferation of the cells, intracellular PA content, and SDH activity in the mitochondria.</p><p><b>CONCLUSION</b>Genipin is involved in the energy metabolism of androgen-independent PC3 prostate cancer cells by reducing the content of intracellular PA and the activity of SDH in the mitochondria, which may be associated with its inhibitory effect on UCP-2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Prostatic Neoplasms
/
Succinate Dehydrogenase
/
RNA, Messenger
/
Pyruvic Acid
/
Mitochondrial Proteins
/
Iridoids
/
Cell Line, Tumor
/
Energy Metabolism
/
Uncoupling Protein 2
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS