Androgen may improve erectile function in castrated rats by regulating the ERK1/2 pathway / 中华男科学杂志
National Journal of Andrology
;
(12): 967-972, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-304789
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of the extracellular signal-regulated protein kinase 1/2 (ERK1/2) pathway in erectile dysfunction (ED) caused by the absence of testosterone (T).</p><p><b>METHODS</b>We randomly divided 30 eight-week-old healthy male SD rats into groups A (control) , B (castration), and C (castration + androgen replacement). The rats in groups B and C were castrated surgically, and those in C injected with T undecanoate (100 mg/kg) at 1 week after castration, while the others with 0.9% normal saline instead. At 1 month after treatment, we determined the serum T level, intracavernous pressure (ICP), and mean carotid arterial pressure (MAP) of the rats, and detected the expressions of ERK1/2 and endothelial nitric oxide synthase (eNOS) by Western blot.</p><p><b>RESULTS</b>The serum T level was significantly lower in group B ([1.27 ± 0.48] nmol/L) than in A ([17.14 ± 1.07] nmol/L) and C ([16.24 ± 1.90] nmol/L) (P < 0.05), and so were ICP and MAP (P < 0.05). The expression of ERK1/2 showed no statistically significant differences among the three groups (P > 0.05), that of phosphatase ERK1/2 was markedly higher while that of eNOS remarkably lower in group B than in A and C (both P < 0.05).</p><p><b>CONCLUSION</b>Androgen replacement may improve the erectile function of castrated rats by regulating the ERK1/2 pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penis
/
Testosterone
/
Penile Erection
/
Orchiectomy
/
Blotting, Western
/
Rats, Sprague-Dawley
/
Mitogen-Activated Protein Kinase 1
/
Hormone Replacement Therapy
/
MAP Kinase Signaling System
/
Therapeutic Uses
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2015
Type:
Article
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