Hydrophidae identification through analysis on Cyt b gene barcode / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3179-3182, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-304837
ABSTRACT
Hydrophidae, one of the precious traditional Chinese medicines, is generally drily preserved to prevent corruption, but it is hard to identify the species of Hydrophidae through the appearance because of the change due to the drying process. The identification through analysis on gene barcode, a new technique in species identification, can avoid the problem. The gene barcodes of the 6 species of Hydrophidae like Lapemis hardwickii were aquired through DNA extraction and gene sequencing. These barcodes were then in sequence alignment and test the identification efficency by BLAST. Our results revealed that the barcode sequences performed high identification efficiency, and had obvious difference between intra- and inter-species. These all indicated that Cyt b DNA barcoding can confirm the Hydrophidae identification.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Molecular Sequence Data
/
Base Sequence
/
China
/
Classification
/
Sequence Analysis, DNA
/
Elapidae
/
Reptilian Proteins
/
Cytochromes b
/
DNA Barcoding, Taxonomic
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article
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