Sirolimus inhibits the differentiation, proliferation and migration of endothelial progenitor cells in vitro / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 1021-1025, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-304984
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of sirolimus on differentiation, proliferation, adhesion and migration of endothelial progenitor cells (EPC) in vitro.</p><p><b>METHODS</b>(1) Mononuclear cells (MNC) were isolated from rat bone marrow by Ficoll density gradient centrifugation and cultured on fibronectin-coated culture dishes with or without sirolimus (0.01 - 100 ng/ml) for 12 days. (2) After 8 days cultured, attached cells were treated with sirolimus (0.1 - 200 ng/ml) or vehicle for various time points (12 h, 24 h, 48 h and 96 h). EPC were identified as adherent cells double positive stained for FITC-UEA-I and DiI-acLDL under laser confocal immunofluence microscope. EPC proliferation, migration were assayed with MTT assay and modified Boyden chamber assay respectively.</p><p><b>RESULTS</b>EPC number differentiated from MNC at 12 days was significantly lower in sirolimus treated cells in a dose-dependent manner than that of vehicle-treated cells. Sirolimus also significantly inhibited the proliferative, migratory and adhesive capacity of EPC in a time and dose dependent manner.</p><p><b>CONCLUSION</b>Present results suggested that sirolimus could inhibit EPC differentiation from MNC and reduce the proliferation, migration and adhesion capacities of EPC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Stem Cells
/
Bone Marrow Cells
/
Cell Differentiation
/
Cell Movement
/
Cells, Cultured
/
Rats, Wistar
/
Sirolimus
/
Cell Biology
/
Endothelial Cells
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2006
Type:
Article
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