Comparison of two different methods to detect HIV antibodies / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Evaluated the chemiluminescence and enzyme-linked immunosorbent assay (ELISA) to detect HIV antibodies, and compared the results, to provide a reference for the selection and clinical application of HIV screening.</p><p><b>METHODS</b>3000 cases of our hospital patients were measured by enzyme-linked immunosorbent assay and chemiluminescence immunoassay, using comfirmming experimental results as gold standards. Comparing sensitivity, specificity and other Indicators.</p><p><b>RESULTS</b>In the diagnosis of HIV infection, enzyme-linked immunosorbent assay and chemiluminescence immunoassay had no significant difference. The positive rate of enzyme-linked immunosorbent assay was 0.93%, while the sensitivity and specificity were 89.66%, 99.93%, the positive rate of chemiluminescence immunoassay was 1.03%, while the sensitivity and specificity were 100%, 99.93%, respectively.</p><p><b>CONCLUSION</b>Both methods are suitable for screening of HIV, having high specificity, and chemiluminescence has greater sensitivity than ELISA.</p>