Development and evaluation of a quantitative double antibodies sandwich ELISA assay for rIFN-alpha1b / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 489-491, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-305000
ABSTRACT
<p><b>OBJECTIVE</b>To develop a double antibody sandwich ELISA assay for quantitative determination of recombinant human interferon alpha1b.</p><p><b>METHODS</b>Mouse monoclonal antibodies with different binding site on rIFN-alpha1b were screened to select optimized candidates as coating and HRP-labeled index antibodies respectively. And a double antibodies sandwich ELISA was assembled; the reliable lower detection limit, specificity, accuracy and reproducibility were evaluated and validated.</p><p><b>RESULTS</b>The quantitative sandwich ELISA had a reliable lower detection limit of 10 ng/ml, with a liner detection range 10-100 ng/ml (R2 = 0.992), variation coefficient inter-plates is less than 10%.</p><p><b>CONCLUSION</b>The developed sandwich ELISA was a sensitive and specific, accuracy and reproducibility method for quantitative determination of recombinant human interferon alpha1b in final product.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Interferon-alpha
/
Methods
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2012
Type:
Article
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