Detection of hepatitis A virus RNA with an improved loop-mediated isothermal amplification assay / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 483-485, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-305002
ABSTRACT
<p><b>OBJECTIVE</b>To establish a novel improved loop-mediated isothermal amplification (LAMP) technique to detect hepatitis A virus (HAV).</p><p><b>METHODS</b>A novel improved LAMP assay based on the addition of an acceleration primer was developed for hepatitis A virus nucleotide acid detection.</p><p><b>RESULTS</b>Precision and reproducibility analysis proved its high stability and reliability. Comparison between the improved and conventional LAMP assays revealed that the former was more sensitive with a detection limit of 5 TCID50/ml. The novel detection method displayed 100% consistency with the TaqMan real-time PCR assay when applied to clinical specimens collected from patients with confirmed acute HAV infection.</p><p><b>CONCLUSION</b>This novel technique is widely applicable as a simple diagnostic tool in the clinical field as well as for the surveillance and investigation of the infectious disease in developing countries where HAV is endemic.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
RNA, Viral
/
DNA Primers
/
Nucleic Acid Amplification Techniques
/
Hepatitis A virus
/
Genetics
/
Hepatitis A
/
Metabolism
/
Methods
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2012
Type:
Article
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