Study on the correlation between membrane protein Flotillin-1 and PrPc endocytosis / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 435-438, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-305016
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether the membrane-associated protein Flotillin-1 has relationship with endocytosis of PrPc.</p><p><b>METHODS</b>The expression of Flotillin-1 in different cell lines was detected with the method of Western Blot; the interaction between Flotillin-1 and PrPc in Cells which were treated with copper ions was observed using immunoprecipitation method.</p><p><b>RESULTS</b>(1) Flotillin-1 was widely expressed in many cell lines without significant difference in the amounts of expression level; (2) Only in the appearance of copper ions, the protein complexes of PrPc and Flotillin-1 can be detected with the method of IP, which were related to copper ions concentration and processing time.</p><p><b>CONCLUSION</b>The membrane-associated protein Flotillin-1 has the relationship with the endocytosis of PrPc.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Cell Line
/
Cell Membrane
/
PrPC Proteins
/
Protein Transport
/
Endocytosis
/
Genetics
/
Membrane Proteins
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS