Analysis of relationship of HBV PreS1 antigen, anti-HBc IgM, DNA load and genotypes in hepatitis B patients / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 328-330, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-305044
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship of HBV PreS1 antigen, anti-HBc IgM, DNA load and genotypes, and the significance for clinical diagnosis and prognostic.</p><p><b>METHODS</b>Enzyme linked immune-sorbent assay was used to test the HBV serum markers of HB patients; HBV-DNA copies was detected by time fluorescence quantitative PCR; using nested PCR to amplify the S fragment of HBV genome, then sequence and make blast with HBV standard sequences to ascertain genotypes. Make comprehensive analysis of these indexes.</p><p><b>RESULTS</b>355 serum specimens of acute or chronic HB patients were collected. The positive rates of HBV PreS1-Ag and HBV-DNA in model I (positive for HBeAg) were 80.2% and 73.7% respectively, which both higher than other models. The abnormal rate of ALT and AST were higher in PreS1-Ag positive group than negative, as well as in anti-HBc IgM positive group. There are 4 samples is genotype B (2.9%), 76 genotype C (55.9%) and 56 genotype D (41.2%). Positive rate of HBeAg and HBV-DNA of genotype C samples were both higher than which of genotype B and D.</p><p><b>CONCLUSION</b>PreS1-Ag and Anti-HBe-IgM indexes are of great value to viral hepatitis B early diagnosis, HBV replication surveillance and prognostic evaluation; the major HBV genotypes in Henan province are C and D, and the positive rate of HBeAg and HBV-DNA were both higher in genotype C HBV infection population than genotype B and D.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Precursors
/
Virology
/
Virus Replication
/
Blood
/
DNA, Viral
/
Immunoglobulin M
/
Viral Load
/
Allergy and Immunology
/
Genotype
/
Hepatitis B
Type of study:
Prognostic study
/
Screening study
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2012
Type:
Article
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