Primary establishment of an alphaLISA assay for detection of HAV IgM / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 139-141, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-305075
ABSTRACT
<p><b>OBJECTIVE</b>To develop an AlphaLISA method for detection of antibody of Hepatitis A virus.</p><p><b>METHODS</b>After hepatitis A virus antigen was concentrated and biotinylated, optimal biotinylated HAV antigen, donor bead, acceptor bead concentration have been explored and determined by both dot-blotting and AlphaLISA methods. 97 samples including 23 serums from patients HAV infected and 70 serums from blood donors have been detected by new AlphaLISA method.</p><p><b>RESULTS</b>The sensitivity and specificity for anti-HAV IgM were 78% and 98.5%, the positive and negative predictive value were 95% and 93%.</p><p><b>CONCLUSION</b>A homogeneous and fast AlphaLISA assay for detection of HAV IgM has been established by preliminary verification on samples.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Immunoglobulin M
/
Sensitivity and Specificity
/
Biotinylation
/
Hepatitis A Antibodies
/
Diagnosis
/
Hepatitis A
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2012
Type:
Article
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