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Preparation and identification of a recombinant hepatitis C virus (HCV) based on sindbis virus vector / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 136-138, 2012.
Article in Chinese | WPRIM | ID: wpr-305076
ABSTRACT
<p><b>OBJECTIVE</b>To construct the recombinant virus-like particles containing HCV envelope glycoprotein E1E2 based on sindbis virus vector.</p><p><b>METHODS</b>The gene encoding HCV envelope glycoprotein E1E2 was cloned into sindbis virus vector to construct recombinant plasmids pBR-XJE1E2 and pVA-XJE1E2, and transfect them into BHK-21 cells to obtain recombinant virus-like particles. The expression of E1 and E2 protein were verified by Western Blot and indirect immunofluorescent assay (IFA).</p><p><b>RESULTS</b>The results of restriction enzyme digestion, PCR and sequencing analysis showed that the recombinant plasmids were constructed successfully. And the results of RT-PCR, Western blotting and IFA detection showed that the transfect cells could package HCV-like particles of expressing structural proteins E1E2.</p><p><b>CONCLUSION</b>The recombinant expression plasmids pBR-XJE1E2 and pVA-XJE1E2 based on sindbis virus vector could package HCV-like particles in eukaryotic cell, which provides a foundation for further study of its in vivo animal immune response.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Recombination, Genetic / Sindbis Virus / Cells, Cultured / Viral Envelope Proteins / Hepacivirus / Genetic Vectors / Genetics Limits: Animals Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Recombination, Genetic / Sindbis Virus / Cells, Cultured / Viral Envelope Proteins / Hepacivirus / Genetic Vectors / Genetics Limits: Animals Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2012 Type: Article