Resistin inhibits rat insulinoma cell RINm5F proliferation / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 43-46, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-305111
ABSTRACT
<p><b>OBJECTIVE</b>Resistin was thought to link the obesity to type 2 diabetes. This study aimed to investigate the effect of resistin on insulinoma cell proliferation.</p><p><b>METHODS</b>pcDNA3.1-resistin was transfected into rat insulinoma cells RINm5F. Cell proliferation was assessed by the MTT assay. The resistin and SOCS3 mRNA levels were assessed by RT-PCR. The total Akt level and the phosphorylation status were assessed by Western blot.</p><p><b>RESULTS</b>The over-expressed resistin inhibited the RINm5F cell proliferation (p<0.05). SOCS-3 expression was up-regulated by resistin over-expression (3.2 folds over the control; p<0.05). Akt phosphorylation was down-regulated by resistin over-expression (0.6 fold over the control; p<0.05).</p><p><b>CONCLUSIONS</b>Resistin impairs the rat insulinoma cell RINm5F proliferation. This might be attributed to a down-regulation of Akt level caused by increased SOCS-3 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreatic Neoplasms
/
Pathology
/
Phosphorylation
/
Physiology
/
Transfection
/
Cell Line, Tumor
/
Cell Proliferation
/
Resistin
/
Proto-Oncogene Proteins c-akt
/
Suppressor of Cytokine Signaling Proteins
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2010
Type:
Article
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