Effects of NYGGF4 gene over-expression on the insulin sensitivity and secretory function of adipocytes / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 846-849, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-305161
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of a new obesity-related gene NYGGF4 on the insulin sensitivity and secretory function of adipocytes.</p><p><b>METHODS</b>3T3-L1 preadipocytes transfected with either an empty expression vector (pcDNA3.1; control group) or an NYGGF4 expression vector (NYGGF4-pcDNA3.1) were cultured in vitro and differentiated into the matured adipocytes with the standard insulin plus dexamethasone plus 3-isobutyl-methylxanthine (MDI) induction cocktail. 2-deoxy-D-[3H] glucose uptake was determined by liquid scintillation counting. Western blot was performed to detect the protein content and translocation of glucose transporter 4 (GLUT4). The supernatant concentrations of TNF-alpha, IL-6, adiponectin and resistin were measured using ELISA.</p><p><b>RESULTS</b>NYGGF4 over-expression in 3T3-L1 adipocytes reduced insulin-stimulated glucose uptake. NYGGF4 over-expression impaired insulin-stimulated GLUT4 translocation without affecting the total protein content of GLUT4. The concentrations of TNF-alpha, IL-6, adiponectin and resistin in the culture medium of 3T3-L1 transfected with NYGGF4 were not significantly different from those in the control group.</p><p><b>CONCLUSIONS</b>NYGGF4 over-expression impairs the insulin sensitivity of 3T3-L1 adipocytes through decreasing GLUT4 translocation and had no effects on the secretory function of adipocytes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Transfection
/
Carrier Proteins
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Adipocytes
/
Bodily Secretions
/
3T3-L1 Cells
/
Adiponectin
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2009
Type:
Article
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