Fermentation, purification and identification of recombinant RGD-Hirudin / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 126-129, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-305215
ABSTRACT
Recombinant RGD-Hirudin ( r-RGD-Hirudin ) has double functions anti-thrombin activity and anti-platelet aggregation activity. To identify these functions, the expression plasmid, RGD-Hirudin-pPIC9K, was constructed by inserting cDNA of RGD-hirudin in yeast expression vector pPIC9K. The high expression clone was gained after screening. This clone was fermented for 3 days. The r-RGD-hirudin was secreted into the culture. It was ultra-filtrated from culture supernatant, then after gel filtration chromatography and anion exchange chromatography, the purified r-RGD-hirudin was gained. Its purity was larger than 97% and its specific activity was 12 000 ATU/mg. The yield per liter culture of purified r-RGD-hirudin was 1 g and overall recovery yield was more than 75% . The purified r-RGD-hirudin was identified by reductive SDS-PAGE, anti-thrombin activity assay, anti-platelet aggregation assay, LC/MS and isoelectrofocusing assay. It is proved that r-RGD-Hirudin is ramification of wt-Hirudin and it has anti-thrombin activity and anti-platelet aggregation activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pichia
/
Recombinant Proteins
/
Platelet Aggregation Inhibitors
/
Hirudins
/
Rats, Sprague-Dawley
/
Fermentation
/
Genetics
/
Molecular Weight
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2004
Type:
Article
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