Co-expression of gpd1 and hor2 from Saccharomyces cerevisiae in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 385-389, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-305264
ABSTRACT
Based on the principle of the pathway engineering, a novel pathway of producing glycerol was built in E. coli. The gpd1 gene encoding glycerol 3-phosphate dehydrogenase and the hor2 gene encoding glycerol 3-phosphatase were cloned from Saccharomyces cerevisiae, respectively. The two genes were inserted into expression vector pSE380 together. A recombinant plasmid pSE-gpd1-hor2 containing polycistron was constructed under the control of the strong trc promoter. Then it was transformed into E. coli BL21. The result showed the recombinant microorganism GxB-gh could convert glucose to glycerol directly. And the recombinant microorganism GxB-gh was incubated to produce glycerol from D-glucose in the fermentor. The maximal concentration of glycerol was 46.67g/L at 26h. Conversion rate of glucose was 42.87%. The study is about "green" producing glycerol by recombinant microorganism and is also useful for further working in recombining microorganism of producing 1,3-propanediol.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Saccharomyces cerevisiae
/
Fungal Proteins
/
Genetic Engineering
/
Cloning, Molecular
/
Phosphoric Monoester Hydrolases
/
Escherichia coli
/
Fermentation
/
Genetics
/
Glycerol
/
Glycerolphosphate Dehydrogenase
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2005
Type:
Article
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