Simultaneous determination of flavones and saponins of Rhizoma Anemarrhenae by HPLC-DAD-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 108-111, 2015.
Article
in Zh
| WPRIM
| ID: wpr-305339
Responsible library:
WPRO
ABSTRACT
This study is to establish an HPLC-DAD-ELSD method for simultaneous determination of 5 flavones and saponins in Rhizoma Anemarrhenae including neo-mangiferin, mangiferin, timosaponin B II, timosaponin B III and timosaponin A III. Samples were analyzed on a Merck Purospher STAR column(4.6 mm x 250 mm, 5 μm). The mobile phase consisted of acetonitrile( A) and 0. 1% formic acid (B) with gradient elution at a flow rate of 1.0 mL · min(-1). The column temperature was set at 40 °C. The DAD detector wavelength was set at 254 nm. The ELSD conditions were as follows: the nebulizing gas flow rate was 2.0 L · min(-1) and temperature of drift tube was 105 °C. The volume was 10 μL. The five compounds were well separated with good linear correlations. The mean recoveries were between 102.0%-104.0%. This method was quick and reliable which provides a foundation for quality control of R. Anemarrhenae.
Full text:
1
Index:
WPRIM
Main subject:
Saponins
/
Drugs, Chinese Herbal
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Chemistry
/
Chromatography, High Pressure Liquid
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Rhizome
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Anemarrhena
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Flavones
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Methods
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article