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Determination of β-sitosterol and total sterols content and antioxidant activity of oil in acai (Euterpe oleracea) / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4620-4624, 2014.
Article in Chinese | WPRIM | ID: wpr-305372
ABSTRACT
In order to establish a method for the determination of the sterols of the oil in the freeze-dried acai (Euterpe oleracea Mart.) and to evaluate its antioxidant activities, a saponification/extraction procedure and high performance liquid chromatography (HPLC) analysis method were developed and validated for the analysis of phytosterols in PEE (Petroleum ether extract). Separation was achieved on a Purosper STAR LP C18 column with a binary, gradient solvent system of acetonitrile and isopropanol. Evaporative light scattering detection (ELSD) was used to quantify β-sitosterol and the total sterols. Peak identification was verified by retention times and spikes with external standards. Standard curves were constructed (r = 0.999 2) to allow for sample quantification. Recovery of the saponification and extraction was demonstrated via analysis of spiked samples. The highest content of total sterols is β-sitosterol. The antioxidant activities of the extracts were evaluated using the total oxyradical scavenging capacity assay (TOSC assay). The result showed that the PEE exhibited significant antioxidant properties, sample concentration and the antioxidant capacity had a certain relevance.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phytosterols / Sitosterols / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Arecaceae / Antioxidants Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phytosterols / Sitosterols / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Arecaceae / Antioxidants Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article