Expression and switching of TH1/TH2 type cytokines gene in human gliomas / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 268-272, 2005.
Article
in English
| WPRIM
| ID: wpr-305403
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and switching of Th1/Th2 cytokines gene in human gliomas and its effects on occurring and developing of human gliomas.</p><p><b>METHODS</b>Interleukin (IL)-2 and interferon-gamma represent Th1 type cytokines. IL-4, IL-6, IL-10, and IL-13 represent Th2 type cytokines. The gene expressions of Thl/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes, and glioma cell lines were detected by reverse transcription polymerase chain reaction (RT-PCR). The biological activity of cytokines in the supernatant of glioma cell lines was assayed by enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The total positive rates of Th1 and Th2 type cytokines gene in human glioma cells were 14.77% and 75%. The total positive rates of Th1 and Th2 type cytokines gene in glioma infiltrating lymphocytes were 22.73% and 68.17%. There was obviously predominant expression of Th2 type cytokines in human glioma tissues, glioma infiltrating lymphocytes, and glioma cell lines. There was no unbalanced expression of Th1/Th2 cytokines in normal brain tissues.</p><p><b>CONCLUSION</b>There is a predominant expression of Th2 type cytokines in human glioma cells. The switching of Thl/Th2 cytokines gene may play an important role in the occurring and developing of human gliomas.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Astrocytoma
/
Brain Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Cytokines
/
Interleukin-4
/
Interleukin-6
/
Interferon-gamma
/
Interleukin-2
/
Interleukin-10
Limits:
Adolescent
/
Adult
/
Aged
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2005
Type:
Article
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