Allele distribution of five X-chromosome short tandem repeat loci in Ewenke population of north China / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 237-241, 2005.
Article
in English
| WPRIM
| ID: wpr-305411
ABSTRACT
<p><b>OBJECTIVE</b>To study the allele genetic polymorphism of five short tandem repeat (STR) loci on X-chromosome in Ewenke population of north China and to provide basic data for forensic identification.</p><p><b>METHODS</b>Genomic DNA was extracted from EDTA-whole blood of Ewenke population by Chelex-100. The DNA samples were amplified by PCR and were analyzed by polyacrylamide gel electrophoresis and silver staining. The sequence length variations of DXS6799, DXS8378, DXS101, HPRTB, and DXS6789 loci on X-chromosome in 98 unrelated Ewenke individuals were investigated.</p><p><b>RESULTS</b>All five loci analyzed showed high polymorphism and genetic stability. The data of the five X-chromosome STR loci in Ewenke ethnic group of China was in accordance with Hardy-Weinberg equilibrium by Chi-square test.</p><p><b>CONCLUSION</b>Allele polymorphism of five X-chromosome STR loci can be used as a genetic marker for forensic identification and population genetic research.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
DNA
/
Genetic Markers
/
China
/
Polymerase Chain Reaction
/
Microsatellite Repeats
/
Chromosomes, Human, X
/
Asian People
/
Ethnology
/
Forensic Medicine
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2005
Type:
Article
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