Regular expression of discoidin domain receptor 2 in the improved adjuvant-induced animal model for rheumatoid arthritis / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 133-137, 2005.
Article
in English
| WPRIM
| ID: wpr-305439
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of discoidin domain receptor 2 (DDR2) of fibroblast-like synovial cells in improved adjuvant-induced animal (AIA) model for rheumatoid arthritis (RA) and to provide evidence for DDR2's antagonist use clinically.</p><p><b>METHODS</b>AIA was modified by administrating 0.1 mL of complete Freund's adjuvant (CFA, mixed with 5 mg Bacillus Calmette-Guerin vaccine/mL) into rats' right hind paws and 0.125 mL tumor necrosis factor-alpha (2 U/mL) into right ankles and subpatellar fatty tissue. The expression of DDR2 in fibroblast-like synovial cells was assessed using immunohistochemistry, immunofluorescence histochemistry, and in situ hybridization methods. Levels of anti-collagen II antibody were measured using enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>Given the terms mentioned above, we found a more practical rat model, apparently decreasing immunization time (average 3-5 days). DDR2 can be detected upon the 15th day of immunization; expression gradually increased with time going on, and reaching a peak 35 days after immunization before gradually decreasing. Serum anti-collagen II antibody showed similar expression patterns as DDR2, but reached peak later than DDR2, about 40 days after immunization.</p><p><b>CONCLUSION</b>Regular expression of DDR2 in animal models infers its important role in the pathological process of RA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Arthritis, Experimental
/
Arthritis, Rheumatoid
/
Synovial Fluid
/
Blood
/
BCG Vaccine
/
Receptors, Mitogen
/
Freund's Adjuvant
/
Rats, Sprague-Dawley
/
Receptor Protein-Tyrosine Kinases
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2005
Type:
Article
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