Effect of oxidized-LDL on NF-kappaB nuclear translocation in aortic smooth muscle cells originated from rats of different ages / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 112-115, 2005.
Article
in English
| WPRIM
| ID: wpr-305446
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanism of atherosclerosis that related to age.</p><p><b>METHODS</b>Immunohistochemistry staining and Western blot were adopted to determine the nuclear translocation of nuclear factor-kappa B (NF-kappaB) and expression of platelet-derived growth factor B (PDGF-B) in smooth muscle cells (SMCs) co-cultured with low density lipoprotein (LDL), oxidized LDL (ox-LDL), and ox-LDL+high density lipoprotein (HDL) originated from rats of 2 and 10 months old respectively. Fat stain was used to identify the lipid intake in SMCs.</p><p><b>RESULTS</b>The optimal stimulation time of ox-LDL to SMCs was 12 hours. NF-kappaB intensity increased in most nuclei of SMCs that originated from rats of either 2 or 10 months old co-cultured with ox-LDL. The intensity of NF-KB and the amount of intracellular lipid taken in SMCs were more obvious in cells from 10-month-old rats than from the younger ones. Change of PDGF-B expression in SMCs was not remarkable in each group of rats.</p><p><b>CONCLUSIONS</b>The 10-month-old rats are more susceptive to ox-LDL than 2-month-old rats in activating nuclear translocation of NF-kappaB. Maybe this is one of the important reasons contributing to the difference between the older and younger rats on the initiation and development of atherosclerosis lesion. Expression of PDGF-B is not associated with the activity of nuclear translocation of NF-kappaB.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta
/
Pharmacology
/
Cell Nucleus
/
Cells, Cultured
/
NF-kappa B
/
Age Factors
/
Rats, Wistar
/
Proto-Oncogene Proteins c-sis
/
Culture Media
/
Cell Biology
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2005
Type:
Article
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