Expression, purification, and characterization of an anti-human RBC ScFv-HIV gp160 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV.</p><p><b>METHODS</b>The gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E. coli.</p><p><b>RESULTS</b>The fusion protein was proved to be able to bind both anti-RBC and HIVgp160. It could cause agglutination of human RBC when HIVgp160 was present.</p><p><b>CONCLUSION</b>The fusion protein has the potential in rapid detection of HIV.</p>