Evaluation of the ELISA diagnostic kits for hepatitis E virus antibody in the reference serum, the suspect patients of hepatitis E and normal persons' sera / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 59-61, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-305495
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the quality of the ELISA diagnostic kits for detecting hepatitis E virus (HEV) specific IgG antibody.</p><p><b>METHODS</b>Five diagnostic kits (WT, GD, HM, GeneLabs and KH) for anti-HEV IgG were assayed by detecting HEV IgG in the HEV diagnostic reference sera from 24 positive cases and 30 negative cases. 42 cases clinical suspect patients with hepatitis E from Ditan hospital in Beijing in 1994 to 1995 and 230 normal persons' sera from Chengdu city, Sichuan province in September in 2005.</p><p><b>RESULTS</b>The conformity rates of positive and negative sera was 95.83% (23) and 96.67% (29) for WT kit, 87.50% (21) and 100% (30) for GD kit, 87.50% (21) and 96.67% (29) for HM kit, 66.67% (16) and 100% (30) for GeneLabs kit, 45.83% (11) and 100% (30) for KH kit for detecting anti-HEV positive and negative reference sera, respectively. The five kits were used to detect anti-HEV IgG among 42 clinical suspect patients with hepatitis E and 230 normal persons' sera, The positive rate was 97.62% (41) and 31.74% (73) by WT, 100% (42) and 17.39% (40) by GD kit, 97.62% (41) and 23.91% (55) by HM kit, 90.48% (38) and 7.83% (18) by GeneLabs kit, 90.48% (38) and 3.48% (8) by KH, respectively.</p><p><b>CONCLUSION</b>The positive rates of all the 5 kits were over 90% and had a very high conformity in detecting anti-HEV IgG in clinical patient with hepatitis E, positive rates of 3.48% to 31.74% were found in detecting the normal persons' sera. The research showed insufficiency of the ELISA kits for epidemiological survey of HEV infection in population.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Kits, Diagnostic
/
Reference Standards
/
Virology
/
Blood
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Hepatitis Antibodies
/
China
/
Reproducibility of Results
/
Sensitivity and Specificity
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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