Association between human papillomavirus (HPV) type 16 infection and E6/E7 gene variant and the cervical lesions in Beijing / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 32-34, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-305504
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the association between HPV16 infection, E6/E7 variations and the cervical lesions.</p><p><b>METHODS</b>HPV subtypes were detected by using flow-through hybridization technique, E6/E7 gene was extracted from cervical lesions in 80 patients with HPV16 infection, PCR amplified, cloned into plasmid pMD18-T and sequenced.</p><p><b>RESULTS</b>HPV 16 was the most common type which accounted for 33.3% (154/463), the HPV16 infection rates increased with the severity of cervical lesions (P < 0.05). Totally in 72 cases the complete E6 and E7 regions were successfully sequenced, the DNA mutation rate of E6/E7 was 88.9% (64/72). A mutation, E6-D32E (T96G) coincided with a specific type of E7 mutation, N29S (A86G). D32E/N29S mutation rate was 38.9% (28/72), the detection rate increased with the severity of cervical lesions (P < 0.05).</p><p><b>CONCLUSION</b>HPV 16 was the most common type in women with cervical lesions in Beijing, D32E/N29S variant associated with the cervical lesions.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Repressor Proteins
/
Virology
/
Severity of Illness Index
/
Uterine Cervical Diseases
/
Uterine Cervical Neoplasms
/
China
/
Oncogene Proteins, Viral
/
Epidemiology
Limits:
Adult
/
Aged
/
Female
/
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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