Immunogenicity of a chimeric adenovirus type 5 vector with type 35 fiber containing HIV-1 gag in mice / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 5-7, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-305513
ABSTRACT
<p><b>OBJECTIVE</b>To study the immune effect of a chimeric adenovirus type 5 vector with type 35 fiber (rAd5/F35) vaccine in BALB/c mice.</p><p><b>METHODS</b>The expression of HIV Gag protein was determined using indirect immunofluorescent staining. The rAd5/F35-mod.gag vector was injected intramuscularly to mice. The IgG antibody was detected by ELISA and CTL response was detected by intracellular cytokine stain assay.</p><p><b>RESULTS</b>The rAd5/F35-mod.gag vector could express HIV Gag protein in vitro and generate strong HIV-specific immune responses in vivo. But anti-Ad5 immunity could limit its immunogenicity in vivo.</p><p><b>CONCLUSION</b>The rAd5/F35-mod.gag vector can elicit specific CTL response and IgG antibody in animal model. In mice with high Ad5 vector-specific immunity, Ad5/F35-mod.gag showed lower level of Gag specific CTL and antibody response than in mice without pre-existing adenovirus type 5 immunity. The results indicated that fiber exchange alone does not evade pre-existing Ad5 immunity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Recombinant Fusion Proteins
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
HIV Antibodies
/
Random Allocation
/
Gene Products, gag
/
Adenoviridae
/
HIV-1
/
Immunization
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS