Identification and typing of human enteroviruses using an RT-PCR assay / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 69-71, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-305522
ABSTRACT
<p><b>BACKGROUND</b>To establish a molecular detection and typing assay for identification and typing of human enteroviruses (HEV) which is suitable for clinical detection and epidemiologic research.</p><p><b>METHODS</b>Using both primers specific for HEV genus and HEV typing primers and reverse transcription polymerase chain reaction (RT-PCR) the authors detected preliminarily HEV by agarose gel electrophoresis and then identified serotype through nucleotide sequence analysis of RT-PCR amplicons. The monospecific antisera neutralization was applied to validate the typing results.</p><p><b>RESULTS</b>The serotype of 18 suspicious HEV samples was identified 4 Coxsackievirus type A24 (CVA24), 3 CVB3, 1 CVB2, 1 CVA9, 1 CVA15, 1 Echovirus type 3 (E3), 1 E6, 1 E9, 1 E11, 1 E14, 1 E33 and 1 Rhinovirus type 9. The result was validated by monospecitive antisera neutralization.</p><p><b>CONCLUSION</b>This RT-PCR assay for HEV detection and typing may be suitable for clinical detection and epidemic research since this method is sensitive and specific for direct identification and typing of HEV.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Viral
/
Serotyping
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Classification
/
DNA Primers
/
Enterovirus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Diagnosis
/
Enterovirus Infections
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2006
Type:
Article
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