Full length nucleotide sequence analysis of L and M segments of Hantavirus Q32 strain gene / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>BACKGROUND</b>To study the full length L and M sequence of Hantavirus Q32 strain gene and explore its molecular characters.</p><p><b>METHODS</b>The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.</p><p><b>RESULTS</b>The L genome segment of Q32 virus was found to be 6533 nucleotides in length. One large open reading frame was found located at bases 38 to 6493. This was predicted to encode an L protein 2151 amino acids in length with a molecular mass of 2.46 x 10(5). The M genome segment was 3616 nucleotides in length. One open reading frame was located at bases 41 to 3488. This was predicted to encode an M protein 1135 amino acids with a molecular mass of 1.26 x 10(5).</p><p><b>CONCLUSION</b>The nucleotides sequence of M and L segments of strain Q32 was similar to that of other Hantavirus M and L segments. Deduced amino acid sequences of glycoprotein and RNA polymerase revealed high homologue to other Hantavirus.</p>