Expression and characterization of transcutaneous immunization adjuvant LTB and LTK63 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 8-11, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-305558
ABSTRACT
<p><b>BACKGROUND</b>To study a new kind of adjuvant transcutaneous immunization adjuvant.</p><p><b>METHODS</b>The full length gene of Heat-labile enterotoxin (LT) was amplified from E. coli H10407. The B subunit protein LTB and the nontoxic A subunit protein LTKA were expressed by genetic engineering manipulation. After purification, they were identified with SDS-PAGE, GM1-ELISA and so on.</p><p><b>RESULTS</b>The LTB protein still persisted its biologic activity that conjugated specifically with GM1 ganglioside, and the LTK63 protein lost its toxin activity.</p><p><b>CONCLUSION</b>The results showed that LTB and LTK63 may be used as promising transcutaneous immunization adjuvant.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Bacterial Toxins
/
Recombinant Fusion Proteins
/
Genetic Engineering
/
Gene Expression
/
Adjuvants, Immunologic
/
Blotting, Western
/
Cricetulus
/
CHO Cells
/
Cloning, Molecular
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2006
Type:
Article
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