Expression of SEPT4 protein in the ejaculated sperm of idiopathic asthenozoospermic men / 中华男科学杂志
National Journal of Andrology
;
(12): 699-702, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-305804
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of the SEPT4 protein in the pathogenesis of idiopathic asthenozoospermia.</p><p><b>METHODS</b>Samples of ejaculated sperm from idiopathic asthenozoospermia patients and normozoospermic men were separated and purified by Percoll discontinuous density gradients, the distribution and expression of SEPT4 in the sperm samples were determined by immunocytochemistry, and the expressions of SEPT4 mRNA and SEPT4 protein were detected by RT-PCR and Western blot.</p><p><b>RESULTS</b>Immunocytochemistry showed that the expression of SEPT4, located in the annulus, was significantly reduced in the sperm of the idiopathic asthenozoospermia patients (t = 3.452, P < 0.01). RT-PCR revealed that the expression of SEPT4 mRNA was significantly lower in the sperm of the idiopathic asthenozoospermia patients than in those of the normozoospermic men (t = 3.521, P < 0.05). Western blot confirmed the results of RT-PCR (t = 5.872, P < 0.05).</p><p><b>CONCLUSION</b>The expression of SEPT4 is significantly decreased in the ejaculated sperm of idiopathic asthenozoospermia patients, which might be one of the causes of idiopathic asthenozoospermia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sperm Motility
/
Spermatozoa
/
Case-Control Studies
/
Asthenozoospermia
/
Septins
/
Metabolism
Type of study:
Observational study
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2011
Type:
Article
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