Core promoter mutations of HBV isolated from patients with chronic hepatitis B in Guangxi / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 477-478, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-305886
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between HBV core promoter mutations and liver damage or HBeAg status.</p><p><b>METHODS</b>Nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA core promoter in 59 sera from patients with chronic hepatitis B in Guangxi, then the HBV DNA positive products were sequenced by direct sequencing.</p><p><b>RESULTS</b>The HBV DNA positive rate of was 59.3%(35/59). All the patients were infected by mutants. The commonest mutation was the double mutation (A --> T at nt1762 and G --> A at nt1764), counting for 57.1% (20/35). The next was C --> G at nt1799, counting for 54.4% (19/35), but this was no function. A --> G at nt1752 (resulting in isoleucine to valine) was seen in 37.1% (13/35) of the HBV DNA positive patients, and T --> C at nt1753 was seen in 20% (7/35). The significant difference in the frequency of T1762A1764 mutant was found between HBeAg positive patients (31.3%) and negative patients (79.0%).</p><p><b>CONCLUSIONS</b>HBV core promoter mutations are common among patients with chronic hepatitis B in Guangxi. T1762A1764 mutant is associated with HBeAg status and chronic hepatitis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Virology
/
Blood
/
Hepatitis B virus
/
Promoter Regions, Genetic
/
Point Mutation
/
Hepatitis B, Chronic
/
Genetics
/
Hepatitis B Core Antigens
/
Hepatitis B e Antigens
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article
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