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Effects of PreS2 synthetic peptides on infiltrating T lymphocyte subgroups expression in hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 415-417, 2003.
Article in Chinese | WPRIM | ID: wpr-305913
ABSTRACT
<p><b>OBJECTIVES</b>To explore whether PreS2 can change the percentage of T lymphocyte subgroups and the ration of CD4+/CD8+ in hepatocellular carcinoma (HCC) caused by HBV.</p><p><b>METHODS</b>The P120-146 region composed by the way of Merrifield, which was the most intensive antigen in PreS2 peptides, served as the antigen after dissolved in 0.01 mol/L PBS. 12 patients were chosed as the subjects, who were pathologically diagnosed as HCC after operation, were HBsAg-, HBeAg-, anti-HBc, and HBV DNA positive in serum, and expressed HBsAg in HCC tissue. The monocytes were isolated and cultured in 96 microplate with 1x 10(6) cells in every well, then the PreS2 synthetic peptides was added in at the doses of 1microg, 5microg, and 10microg, also IL-2 with 500 U was added in. Seven days later, the percentage of CD3+, CD4+, CD8+, and the ratio of CD4+/CD8+ were detected.</p><p><b>RESULTS</b>It was found that the percentage of CD4+ increased significantly (t = 3.508, P < 0.01), and the ratio of CD4+/CD8+ decreasedly obviously (t = 2.235, P < 0.05) in the 5microg PreS2 synthetic peptides group, compared with those in the control group. The percentage of CD3+ rised markedly in the IL-2 group, compared with that in the control group.</p><p><b>CONCLUSION</b>With proper doses, PreS2 is capable of changing the expression of T lymphocyte subgroups in HCC tissue, increasing the percentage of CD4+ obviously and changing the motionless state of CD8+, to make the carcinoma cell killed through the action of CD4+ and CD8+.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Peptide Fragments / Pharmacology / Protein Precursors / Molecular Sequence Data / Lymphocytes, Tumor-Infiltrating / Amino Acid Sequence / CD4-CD8 Ratio / Carcinoma, Hepatocellular / Therapeutic Uses / Dose-Response Relationship, Drug Limits: Humans Language: Chinese Journal: Chinese Journal of Hepatology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Peptide Fragments / Pharmacology / Protein Precursors / Molecular Sequence Data / Lymphocytes, Tumor-Infiltrating / Amino Acid Sequence / CD4-CD8 Ratio / Carcinoma, Hepatocellular / Therapeutic Uses / Dose-Response Relationship, Drug Limits: Humans Language: Chinese Journal: Chinese Journal of Hepatology Year: 2003 Type: Article