In vitro antitumor immune response induced by fusion of dendritic cells and Ewing's sarcoma cells / 中华外科杂志
Chinese Journal of Surgery
;
(12): 803-806, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-306205
ABSTRACT
<p><b>OBJECTIVE</b>Human Ewing sarcoma A673 cells and human peripheral blood-derived DCs were fused to induce an antitumor activity against human EW.</p><p><b>METHODS</b>EW A673 cells and human peripheral blood-derived DCs were fused with polyethylene glycol (PEG).</p><p><b>RESULTS</b>Mature DCs with highly expressed surface markers (CD80, CD86, CD83 and HLA-DR) were generated in vitro and flow cytometry. It showed that the highest fusion efficiency was 23.01%. T cell proliferation assay indicated that the novel dendritomas in fused DCs/A673 cells were the most potent in activation of autologous T cell proliferation. The IFN-gamma assay showed that The IFN-gamma secretion by CTLs activated by the novel dendritomas increased more than by other stimulators. CTL assay demonstrated that the novel dendritomas induced A673 cell-specific cytotoxic responses to lyse the A673 cells in the context of MHC class I.</p><p><b>CONCLUSION</b>The data indicates that fusion of tumor cells with DCs is an attractive strategy to induce tumor rejection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Sarcoma, Ewing
/
In Vitro Techniques
/
Dendritic Cells
/
T-Lymphocytes, Cytotoxic
/
Cell Fusion
/
Cancer Vaccines
/
Cytotoxicity, Immunologic
/
Allergy and Immunology
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2005
Type:
Article
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