Anti-pancreatic cancer effects of human peripheral gammadeltaT cells in a mouse tumor model / 中华外科杂志
Chinese Journal of Surgery
;
(12): 726-729, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-306221
ABSTRACT
<p><b>OBJECTIVE</b>To explore the adoptive immunotherapy effect of peripheral gammadeltaT cells in pancreatic cancer nude mice model.</p><p><b>METHODS</b>Thirty BALB/c nude mice were inoculated subcutaneously 5 x 10(5) Cap-1 cells to regularly developed hypodermal tumors, and then divided into 3 groups randomly, gammadeltaT cells, alphabetaT cells and control group. 2.5 x 10(6) gammadeltaT cells or alphabetaT cells or 100 microl RPMI-1640 were respectively injected into abdominal cavity of mice, combined with 10(4) U rhIL-2 for 3 times. Tumor volume, the survival rate and anti-carcinogenic rate of three groups were compared.</p><p><b>RESULTS</b>Eight control nude mice developed hypodermal tumors, which progressively increased in size, and animals had a mean survival of 88 d. Nine nude mice in gammadeltaT cells group and eight in alphabetaT cells group developed tumors (P > 0.05). Tumor growth was arrested and tumor size was reduced remarkably in gammadeltaT cells group. Mean survival was increased to 113 d with less rate of tumor metastasis and more cases of tumor necrosis in gammadeltaT cells group when compared with alphabetaT cells group and controls.</p><p><b>CONCLUSIONS</b>The anti-tumor effects of gammadeltaT cells against pancreatic cancer are better than those of alphabetaT cells and control groups, and might be promising in the adoptive immunotherapy of pancreatic cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreatic Neoplasms
/
Pathology
/
Therapeutics
/
T-Lymphocytes
/
Immunotherapy, Adoptive
/
Lymphocytes, Tumor-Infiltrating
/
Allergy and Immunology
/
Methods
/
Mice, Nude
/
Neoplasm Transplantation
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2005
Type:
Article
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