The Characterization of T Cell - Derived Neutrophil Survival Factor % / 대한면역학회지
Korean Journal of Immunology
;
: 363-374, 1997.
Article
in Korean
| WPRIM
| ID: wpr-30626
ABSTRACT
It has been reported that the stimulated T lymphocytes might secret a neutrophil survival factor. Thus the goal of study was to determine which molecules are the neutrophil survival factors secreted from the phytohaemagglutinin (PHA)-stimulated T lymphocytes. Human peripheral blood T lymphocytes and neutrophils were isolated by Ficoll-paque density sedimentation from heparinized blood of healthy adult donors. The purity of T lymphocytes and neutrophils were more than 90% and 95%, respectively. The maximal effective condition for the neutrophil viability-sustaining activity was 1 ug/ml af PHA in 12 hours incubation with T lymphocytes. The effect of PHA-stimulated T lymphocyte conditioned medium (TCM) on the neutrophils were used for the comparison with PHA-nonstimulated TCM or enriched medium alone. Neutrophil viability-sustaining activity with PHA-stimulated TCM for 24 hours incubation was significantly higher than other groups (85+/-11 vs 43+/-5 vs 916%; p<0.01). In the analysis of the primary data, the good candidates for the neutrophil viability-sustaining factor were granulocyte/monocyte colony stimulating factor (GM-CSF) and interleukin-8 (IL-8). They were used in the bioassay and antibody neutralization of cytokine activity. ...continue...
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tissue Donors
/
Biological Assay
/
Heparin
/
Lymphocytes
/
T-Lymphocytes
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Interleukin-8
/
Colony-Stimulating Factors
/
Culture Media, Conditioned
/
Neutrophils
Limits:
Adult
/
Humans
Language:
Korean
Journal:
Korean Journal of Immunology
Year:
1997
Type:
Article
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