Desmoplakin expression silencing affects cardiac voltage-gated sodium channel Nav1.5 in HL-1 cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 983-989, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-306425
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the association of desmoplakin with the distribution and function of Nav1.5 by RNA silencing technology in HL-1 cells.</p><p><b>METHODS</b>HL-1 cells with desmoplakin expression suppression by RNA silencing were examined for desmoplakin and Nav1.5 protein expressions by Western blotting, and the distribution and co-location of desmoplakin and Nav1.5 protein were detected by immunofluorescence staining. Patch-clamp recording was applied to analyze the changes in whole-cell sodium current after desmoplakin silencing.</p><p><b>RESULTS</b>Compared with the untreated group and negative control group, the cells with desmoplakin silencing showed obviously reduced expressions of desmoplakin and Nav1.5 proteins. Co-localization of desmoplakin and Nav1.5 was detected at cell-cell contact in untreated and control conditions, and desmoplakin expression silencing induced a drastic redistribution of Nav1.5 with decreased peak current density (156.3∓6.2 vs 41.8∓3.1, n=6, P<0.05), a shift in voltage dependence of steady-state inactivation (-42 mV vs -61 mV, n=5, P<0.05), and prolonged time of recovery from inactivation.</p><p><b>CONCLUSION</b>Desmoplakin silencing caused redistribution of Nav1.5 protein and also changes in its electrophysiological properties in HL-1 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Line
/
Gene Silencing
/
Myocytes, Cardiac
/
Desmoplakins
/
Genetics
/
Metabolism
/
Mutation
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
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