Celecoxib enhances chemosensitivity of oral cancer cells by blocking cell cycle progression in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 885-888, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-306447
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of celecoxib in enhancing the chemosensitivity of oral cancer cells and the correlation of this effect with cell cycle arrest.</p><p><b>METHODS</b>KB/VCR cell line was treated with celecoxib (10, 20, 40, and 80 µmol/L) and/or VCR (0.375, 0.75, 1.5, and 3 µmol/L), and the growth inhibition rates of KB/VCR cells were assessed with MTT assay. Flow cytometry was employed to analyze the distribution of cell cycle. Western blotting was performed to detect the expression of P-glycoprotein (P-gp) and the cell cycle related proteins Cyclin D1 and p21(WAF1/CIP1).</p><p><b>RESULTS</b>Low concentrations of celecoxib (<20 µmol/L) produced no obvious effect on the proliferation of the cells. But at 10 µmol/L, celecoxib significantly enhanced the toxicity of VCR in a time-dependent manner, and the combined treatments for 24, 48, and 72 h caused growth inhibition rates of (37.53∓2.05)%, (46.67∓3.17)% and (54.02∓1.53)%, respectively, significantly higher than those following treatments with celecoxib or VCR alone (P<0.01). Compared with the cells treated with VCR alone , the cells with combined treatments showed a significantly increased cell percentage in G0/G1 phase [(56.08∓0.46)%] with decrease percentages in S phase [(22.83∓0.20)%] and G2/M phase [(21.09%∓0.66)%]. The combined treatment also significantly down-regulated cyclin D1, up-regulated p21(WAF1/CIP1), and reduced P-gp expressions in the cells.</p><p><b>CONCLUSIONS</b>Celecoxib enhances the chemosensitivity of KB/VCR cells by down-regulating P-gp expression, which is partially mediated by modification of cyclin D1 and p21(WAF1/CIP1) to result in cell cycle arrest.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pyrazoles
/
Sulfonamides
/
Mouth Neoplasms
/
KB Cells
/
Cell Cycle
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Neoplasm
/
Cyclin D1
/
Cell Line, Tumor
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
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