Role of muscarinic cholinergic receptor subtypes in regulating glutamatergic synaptic transmission in rat spinal dorsal horn / 南方医科大学学报
Journal of Southern Medical University
;
(12): 838-841, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-306458
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of muscarinic cholinergic receptor (mAChR) subtypes in the regulation of glutamatergic input to the spinal dorsal horn neurons and the possible mechanism.</p><p><b>METHODS</b>Whole-cell voltage-clamp recordings on acute spinal slice was utilized to investigate the effect of activation of mAChRs and blockade of M2/M4 subtypes on glutamatergic synaptic transmission in rat spinal dorsal horn neurons.</p><p><b>RESULTS</b>The nonselective mAChRs agonist oxotremorine-M concentration-dependently decreased the amplitude of monosynaptic and polysynaptic evoked glutamate-mediated excitatory postsynaptic currents (eEPSCs) in most of the neurons. The M2/M4 antagonist himbacine completely blocked the inhibitory effect of oxotremorine-M in 92.3% of monosynaptic and 75% of polysynaptic neurons in the spinal cord slices. In the remaining 16% neurons, himbacine partially blocked the inhibitory effect of oxotremorine-M.</p><p><b>CONCLUSIONS</b>Activation of mAChRs in the spinal cord attenuates synaptic glutamate release to the dorsal horn neurons mainly through M2 and M4 receptor subtypes, indicating that a presynaptic inhibition in the spinal cord may be involved in the regulation of nociception by the cholinergic system and mAChRs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Receptors, Muscarinic
/
Rats, Sprague-Dawley
/
Synaptic Transmission
/
Neurotransmitter Agents
/
Excitatory Postsynaptic Potentials
/
Posterior Horn Cells
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
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