Expression pattern of hsa-miR-9 and its association with BCL6 in EBV-positive and EBV-negative Burkitt lymphoma cell lines / 南方医科大学学报
Journal of Southern Medical University
;
(12): 661-666, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-306491
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differential expression pattern of hsa-miR-9 between EBV-positive and -negative Burkitt lymphoma cell lines and its association with BCL-6.</p><p><b>METHODS</b>The expression of hsa-miR9 and BCL-6 mRNAs in EBV(+) Raji and EBV-Ramous cells in mRNA levels were detected using fluorescence quantitative PCR (QRT-PCR). The two cells lines were transiently transfected with hsa-mir9-inhibitor and hsa-mir9-minicsvia Oligofectamine 2000, and the changes in BCL6 expressions was detected using QRT-PCR and Western blotting. Annexin V/PI staining was used to analyze the apoptosis and morphological changes of the transfected cells.</p><p><b>RESULTS</b>The expression of Hsa-miR9 and BCL-6 was significantly higher in EBV(+) Raji cells than EBV(-) Ramous cells (P<0.01). BCL-6 mRNA and protein expression was reduced in EBV(+) Raji cells after transfection with hsa-miR9-inhibitor but up-regulated in EBV(-) Ramous cells transfected with hsa-miR9-minics. Flow cytometry revealed a significantly decreased apoptosis rate in EBV(+) Raji cells transfected with hsa-miR9-inhibitor but an increased rate in EBV(-) Ramous cells transfected with hsa-miR9-minics, and the results were confirmed by microscopic observations.</p><p><b>CONCLUSION</b>Hsa-miR9 positively regulate the expression of BCL-6 and apoptosis of EBV(+) Raji cells and EBV(-) Ramous cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Virology
/
Transfection
/
Gene Expression Regulation, Neoplastic
/
Cell Division
/
Burkitt Lymphoma
/
Apoptosis
/
Herpesvirus 4, Human
/
MicroRNAs
/
Cell Line, Tumor
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
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