Comparison of two nucleic acid extraction methods for norovirus in oysters / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore a convenient and effective method for norovirus nucleic acid extraction from oysters suitable for long-term viral surveillance.</p><p><b>METHODS</b>Two methods, namely method A (glycine washing and polyethylene glycol precipitation of the virus followed by silica gel centrifugal column) and method B (protease K digestion followed by application of paramagnetic silicon) were compared for their performance in norovirus nucleic acid extraction from oysters. Real-time RT-PCR was used to detect norovirus in naturally infected oysters and in oysters with induced infection.</p><p><b>RESULTS</b>The two methods yielded comparable positive detection rates for the samples, but the recovery rate of the virus was higher with method B than with method A.</p><p><b>CONCLUSION</b>Method B is a more convenient and rapid method for norovirus nucleic acid extraction from oysters and suitable for long-term surveillance of norovirus.</p>